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One purpose of the Algae push is to produce usable Lipids (fats/oils) by using light, co2 and fertilizer. What if one could eliminate the need for light? Eliminate the need for new equipment and work a new function right into the current Ethanol Industry?? Use a secondary fermentation phase. Use a different yeast type (False yeast fungus (Rhodotorula glutinis) that protects itself from light by developing a red crust, produces lipids like algae without all the hassle and expense. On a level the SkunK can understand - is this what the patent application below proposes? More speculation at the end of this blog. . .
If you go to the Official US Patent Application search page and enter "winsness" in the term 1 search query. You will find a new patent application here:
http://appft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&co1=AND&d=PG01&s1=winsness&OS=winsness&RS=winsness
and you can also find it here:
http://www.faqs.org/patents/app/20100028484
This is dated February 4, 2010! This sounds like quite a unique way to increase yields at Ethanol Plants. ". . . yields greater than the theoretical mass of lipids of the grains itself. . ." Are we also on the cutting edge here? SkunK is still reading through this . . .
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"Methods for producing a lipid rich product from a feedstock utilized in wet and dry milling processes for producing ethanol, the method include mixing a culture of lipid producing microorganisms with the feedstock, wherein the feedstock includes co-products of ethanol production and/or biomass; producing lipids within the lipid producing microorganisms; lysing the microorganisms; and isolating the lipid rich product.
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6. The method of claim 5, wherein the fermentation step comprises mixing the lipid producing microorganisms with a fraction to produce a lipid rich product.
7. The method of claim 1, further comprising conditioning the feedstock prior to mixing the culture of the lipid producing microorganism with the feedstock.
8. The method of claim 7, wherein the conditioning comprises steam explosion, autohydrolysis, ammonia fiber expansion, acid hydrolysis, ultrasonication, irradiation (for example, with microwave bombardment, or directed electromagnetic stimulation), hydrodynamic shock, cavitation, enzymatic conditioning, or combinations thereof.
**********
"The lipid producing microorganisms consume and convert the non-ethanol co-products of to lipids. In this manner, yields greater than the theoretical mass of lipids of the grains itself can be obtained by conversion of the non-ethanol co-product from the respective milling process. Advantageously, and as will be discussed herein, the introduction of the lipid microorganisms can utilize the existing infrastructure utilized in ethanol production facilities so as to make the process commercially viable. No significant equipment and/or capital costs are needed to integrate the introduction of the lipid producing microorganisms in the dry, wet or fractionated milling processes. The production of additional lipids from within the existing co-products and recovery of these lipids further increases the output on a per bushel of feedstock basis while decreasing the energy consumption to produce a given volume of fuel."
Like this fish I found in a Japanese Fish market, sometimes you just need a picture to really grasp it. You don't "have" to eat it, but its fun to look. The SkunK put the pictures/diagrams on google docs below:p.2 http://docs.google.com/leaf?id=0B_ch8gAs4lCcMGM4NThiYWItNjk2MS00ZDZjLWJiYTgtMTMwMTAxN2JkN2Fl&hl=en
p.3 http://docs.google.com/leaf?id=0B_ch8gAs4lCcZTYzYTc4NTQtNjM4YS00Mjk0LTk0MmMtMTJkYmEyMTU1YzQ1&hl=en
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Notice: In the patent - lipid producing microorganisms comprise "Rhodotorula glutinis"
Notice the Secondary Fermentation Phase in the above diagrams.
Note this abstract in the African Journal of Biotechnology from 2007 the SkunK found:
****************
ABSTRACT
This study explored a strategy to convert agricultural and forestry residues into microbial lipid, which could be further transformed into biodiesel. Among the 250 yeast strains screened for xylose assimilating capacity, eight oleaginous yeasts were selected by Sudan Black B test. The lipid content of these 8 strains was determined by soxhlet extraction method. One strain (T216) was found to produce lipids up to 36.6%, and it was identified as Rhodotorula glutinis. The optimal fermentation conditions were obtained as follows: glucose as carbon source 100 g/L; yeast extract and peptone as nitrogen sources at, respectively, 8 and 3 g/L; initial pH of 5.0; inoculation volume of 5%; temperature at 28oC, shaking speed of 180 r/min, cultivated for 96 h. Under these conditions, R. glutinis accumulated lipids up to 49.25% on a cellular biomass basis and the corresponding lipid productivity reached 14.66 g/L. Experiments with a 5-L bioreactor under the optimal culture conditions showed that R. glutinis accumulated lipids up to 60.69%, resulting in 23.41 g/L in lipid productivity. More encouraging results were observed for the lipid production with alternative carbon sources. Corn stalk and Populus euramevicana leaves hydrolysate could be used to substitute glucose. Chemical analysis indicated that biodiesel obtained by transesterification possessed similar composition to that from vegetable oil, one of the widely used feedstock for biodiesel.
A conclusion:
In this study, we proposed a technology that has potential to greatly reduce the price of lipid production, which can be used to produce biodiesel and therefore relieve the potential damage of energy crisis. We are optimistic for biodiesel production from lignocellulosic materials although it appears not feasible today.
http://www.bioline.org.br/request?jb07378
**************************
DID GERS and their engineers manage to solve this? Is it feasible today? Hopefully we will hear more about this soon. We will see. . .
SkunK
EXTRA:
GreenShift Mention in March Ethanol Producer Mag:http://ethanolproducer.com/article.jsp?article_id=6359
If you go to the Official US Patent Application search page and enter "winsness" in the term 1 search query. You will find a new patent application here:
http://appft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&co1=AND&d=PG01&s1=winsness&OS=winsness&RS=winsness
and you can also find it here:
http://www.faqs.org/patents/app/20100028484
This is dated February 4, 2010! This sounds like quite a unique way to increase yields at Ethanol Plants. ". . . yields greater than the theoretical mass of lipids of the grains itself. . ." Are we also on the cutting edge here? SkunK is still reading through this . . .
***********
"Methods for producing a lipid rich product from a feedstock utilized in wet and dry milling processes for producing ethanol, the method include mixing a culture of lipid producing microorganisms with the feedstock, wherein the feedstock includes co-products of ethanol production and/or biomass; producing lipids within the lipid producing microorganisms; lysing the microorganisms; and isolating the lipid rich product.
***********
6. The method of claim 5, wherein the fermentation step comprises mixing the lipid producing microorganisms with a fraction to produce a lipid rich product.
7. The method of claim 1, further comprising conditioning the feedstock prior to mixing the culture of the lipid producing microorganism with the feedstock.
8. The method of claim 7, wherein the conditioning comprises steam explosion, autohydrolysis, ammonia fiber expansion, acid hydrolysis, ultrasonication, irradiation (for example, with microwave bombardment, or directed electromagnetic stimulation), hydrodynamic shock, cavitation, enzymatic conditioning, or combinations thereof.
**********
"The lipid producing microorganisms consume and convert the non-ethanol co-products of to lipids. In this manner, yields greater than the theoretical mass of lipids of the grains itself can be obtained by conversion of the non-ethanol co-product from the respective milling process. Advantageously, and as will be discussed herein, the introduction of the lipid microorganisms can utilize the existing infrastructure utilized in ethanol production facilities so as to make the process commercially viable. No significant equipment and/or capital costs are needed to integrate the introduction of the lipid producing microorganisms in the dry, wet or fractionated milling processes. The production of additional lipids from within the existing co-products and recovery of these lipids further increases the output on a per bushel of feedstock basis while decreasing the energy consumption to produce a given volume of fuel."
Like this fish I found in a Japanese Fish market, sometimes you just need a picture to really grasp it. You don't "have" to eat it, but its fun to look. The SkunK put the pictures/diagrams on google docs below:p.2 http://docs.google.com/leaf?id=0B_ch8gAs4lCcMGM4NThiYWItNjk2MS00ZDZjLWJiYTgtMTMwMTAxN2JkN2Fl&hl=enp.3 http://docs.google.com/leaf?id=0B_ch8gAs4lCcZTYzYTc4NTQtNjM4YS00Mjk0LTk0MmMtMTJkYmEyMTU1YzQ1&hl=en
****************
Notice: In the patent - lipid producing microorganisms comprise "Rhodotorula glutinis"
Notice the Secondary Fermentation Phase in the above diagrams.
Note this abstract in the African Journal of Biotechnology from 2007 the SkunK found:
****************
ABSTRACT
This study explored a strategy to convert agricultural and forestry residues into microbial lipid, which could be further transformed into biodiesel. Among the 250 yeast strains screened for xylose assimilating capacity, eight oleaginous yeasts were selected by Sudan Black B test. The lipid content of these 8 strains was determined by soxhlet extraction method. One strain (T216) was found to produce lipids up to 36.6%, and it was identified as Rhodotorula glutinis. The optimal fermentation conditions were obtained as follows: glucose as carbon source 100 g/L; yeast extract and peptone as nitrogen sources at, respectively, 8 and 3 g/L; initial pH of 5.0; inoculation volume of 5%; temperature at 28oC, shaking speed of 180 r/min, cultivated for 96 h. Under these conditions, R. glutinis accumulated lipids up to 49.25% on a cellular biomass basis and the corresponding lipid productivity reached 14.66 g/L. Experiments with a 5-L bioreactor under the optimal culture conditions showed that R. glutinis accumulated lipids up to 60.69%, resulting in 23.41 g/L in lipid productivity. More encouraging results were observed for the lipid production with alternative carbon sources. Corn stalk and Populus euramevicana leaves hydrolysate could be used to substitute glucose. Chemical analysis indicated that biodiesel obtained by transesterification possessed similar composition to that from vegetable oil, one of the widely used feedstock for biodiesel.
A conclusion:
In this study, we proposed a technology that has potential to greatly reduce the price of lipid production, which can be used to produce biodiesel and therefore relieve the potential damage of energy crisis. We are optimistic for biodiesel production from lignocellulosic materials although it appears not feasible today.
http://www.bioline.org.br/request?jb07378
**************************
DID GERS and their engineers manage to solve this? Is it feasible today? Hopefully we will hear more about this soon. We will see. . .
SkunK
EXTRA:
GreenShift Mention in March Ethanol Producer Mag:http://ethanolproducer.com/article.jsp?article_id=6359
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